By M. Cristina Vega
This ebook offers complex expression applied sciences for the construction of protein complexes. due to the fact complexes lie on the middle of recent biology, the expression, purification, and characterization of huge quantities of top quality protein complexes is important for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to advanced reconstitution from person subunits, this ebook bargains important insights and assistance for profitable protein expressionists.
Across a number of sections readers will become aware of present possibilities for the creation of protein complexes in bacterial structures (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary method. a final part introduces in short a number of the biophysical and structural characterization strategies for macromolecular complexes utilizing cutting-edge resolution scattering and nuclear magnetic resonance.
This paintings is a guided travel over the most strong and winning protein expression applied sciences, with a spotlight on co-expression and high-throughput functions. it's addressed to every person drawn to the construction and characterization of macromolecular complexes, from college scholars who wish an available description of the foremost co-expression structures to researchers in biomedicine and the lifestyles sciences looking for an updated survey of obtainable technologies.
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Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega